The SSR Null Allele Problem, and Its Consequences in Pedigree Reconstruction and Population Genetic Studies in Viticulture

Null alleles are that recessive to codominant markers without any effect on the phenotype. In SSR assays, there several reasons for lack of amplification at a locus: primer does not bind well, longer fragments do amplify due imperfections in PCR reaction, or amount DNA sample is insufficient. microsatellite studies, null mostly used pedigree analysis and population genetics calculations such as diversity estimation. can cause rejection true parent; if recognized while they distort results underestimating diversity. this review, effects caused by null-alleles viticultural research its possible solutions were summarized.